Agar media

Question

Having made my agar powder into solution it will not melt again at 50oC, as required for a practical. How do I re-melt it?

 

Answer

This is a feature which is typical of agar media.
 Although it is a gel at room temperature and solidifies at around 30-40°C, it will remain firm at temperatures as high as 65°C or so and will often not melt again fully until the temperature reaches 85°C to 95oC (depending on type). This property is known as hysteresis.
 What you will probably need to do is to bring the solidified agar up to 85-95oC then allow it cool to 50oC, maintaining it there, at which temperature it should remain liquid. All this can be done in a water bath or 2 baths at different temperatures (or heat in a pan of water until it melts, then maintain at 50oC in a water bath).


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Nutrient Agar in Petri Dishes

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